Advance Publication Experimental Animals
نویسندگان
چکیده
20 Isoflurane is a representative inhalant anesthesia used in laboratory animals. 21 However, isoflurane mediates respiratory depression and adverse clinical reactions 22 during induction. In the present study, we established a novel balanced anesthesia 23 method in mice that combined isoflurane anesthesia with midazolam and butorphanol 24 (MB). 25 Thirty-four male C57BL/6J mice received either isoflurane alone or isoflurane with 26 an intra-peritoneal MB premedication (3 mg/kg midazolam and 4 mg/kg butorphanol). 27 The minimum alveolar concentration (MAC) in each group was evaluated. Induction 28 time and adverse clinical reactions were recorded in each group. Core body 29 temperature, heart rate, respiratory rate, and oxygen saturation (SPO2) were assessed 30 before and for 1 h after induction. 31 Premedication with MB achieved a significant reduction in MAC compared with 32 isoflurane monoanesthesia (isoflurane, 1.38 ± 0.15%; isoflurane with MB, 0.78 ± 0.10%; 33 p < 0.05). Induction time was significantly shortened with MB premedication, and 34 adverse reactions such as excitement or incontinence were observed less frequently. 35 Furthermore, isoflurane anesthesia with MB premedication caused increase of 36 respiratory rates compared to isoflurane monoanesthesia. No significant decrease of 37 SPO2 was observed in MBI anesthesia, while a decrease in SPO2 was apparent with 38 isoflurane monoanesthesia (baseline, 98.3% ± 1.1; 10 min after induction, 91.8 ± 6.4%; 39 p < 0.05). 40 In conclusion, premedication with MB was effective for the mitigation of respiratory 41 depression induced by isoflurane in mice, with rapid induction and fewer adverse 42 clinical reactions. 43
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